stress, cont.

I'm getting continued mileage out of this old post,
http://gathatoulie.blogspot.co.uk/2011/01/like-chronically-stressed-rat-looking.html
to which this supplement applies:

"Social defeat was based on the resident-intruder paradigm. In brief,
15 min after the female had been removed from the resident's cage, the experimental male (intruder) was placed inside. The animals were allowed to interact for a maximum of 10 min but usually experimental subjects took no more than 3-5 min to be defeated by the residents, as indicated by the overall behavior and submissive posture (escape, freezing, defensive upright, vocalization). Immediately afterwards, the intruder was physically separated from the resident by an acrylic divider with holes within the resident cage for further 60 min. To avoid individual differences in intensity of defeat, each day the intruders were confronted with another resident, randomized to maximize the time between repeated encounters. In forced swimming, animals were placed inside a 20-cm diameter cylinder half-filled with 24 ± 1oC water during 10 min. Regarding restraint stress, rats were immobilized inside sized- fit PVC tubes for 30 min." --
http://www.sciencemag.org/content/suppl/2009/07/30/325.5940.621.DC1/Dias-Ferreira.SOM.pdf

I'm also surprised that I didn't anywhere note the connection to
http://gathatoulie.blogspot.co.uk/2010/10/country-doctor-effekt.html